Biofact taq polymerase
WebConcentration: 5 units/μl. Description: Taq Core Kit contains all reagents required for PCR (except template and primer) in one box combining simple handling with high flexibility. Taq Polymerase is recommended for routine PCR applications (up to 4 kb fragment length), high throughput PCR or genotyping. The buffer system guarantees robust and ... WebJun 10, 2024 · We performed PCR using BioFACT™ Taq DNA Polymerase (Biofact, Daejeon, Korea). The 25 µL PCR mixture was composed of 5 µL of 10× DNA polymerase buffer, 2.5 units of 10× Taq DNA polymerase, 1 µL of genomic DNA, 10 pmol of each primer, and 0.5 µL of a 0.2 M dNTP mixture. The PCR conditions were as follows: …
Biofact taq polymerase
Did you know?
WebThe Township of Fawn Creek is located in Montgomery County, Kansas, United States. The place is catalogued as Civil by the U.S. Board on Geographic Names and its elevation … WebBioFACT™ A-Star Taq DNA Polymerase는 Thermus aquaticus DNA polymerase 유전자를 E.coli에서 발현하여 고순도로 정제한 recombinant thermostable Taq DNA polymerase에 anti-Taq monoclonal antibody를 결합시킨 Hot Start PCR용 enzyme 입니다. 고온에 도달하기 전까지 antibody가 효소활성을 억제하고 있으며 ...
WebTaq is designed for use in primer extension reaction. Taq can also be used for sequencing. DNA sequencing at high temperature may decrease the second structure of some DNA templates and permit polymerization through base‐ paired region. DNA sequencing with Taq DNA Polymerase produces uniform bands intensities and low background. Performance ... WebAug 17, 1999 · The Taq DNA polymerase is the most commonly used enzyme in DNA sequencing. However, all versions of Taq polymerase are deficient in two respects: (i) these enzymes incorporate each of the four dideoxynucleoside 5′ triphosphates (ddNTPs) at widely different rates during sequencing (ddGTP, for example, is incorporated 10 times …
WebProduct Details. TaqIT DNA Polymerase is an exonuclease deficient derivative of Taq DNA polymerase. The enzyme is supplied in 20 mM Tris-HCl, 222 mM (NH 4) 2 SO 4, 10 mM … WebTaq polymerase is a thermostable DNA polymerase I named after the thermophilic eubacterial microorganism Thermus aquaticus, from which it was originally isolated by …
WebJun 3, 2024 · Taq polymerase is optimally active at 72°C, which is why the extension step is carried out at that exact temperature. In addition, for the proper functioning of Taq polymerase, Mg 2+ is added in the form of …
WebTaq DNA Polymerase is a combination of next-generation polymerase and a novel buffer system that has been designed to deliver very high yield PCR amplification over a wide … can diabetes cause mental health problemsWebreleasing fully active Taq DNA polymerase. This enables specific and efficient primer extension with the convenience of room temperature reaction assembly. Reagent Kit Size Volume Description iTaq DNA polymerase 250 U 50 µl iTaq DNA polymerase, 5 U/µl (Clear Cap) 10X iTaq Buffer 1.25 ml 10X PCR buffer, 200 mM Tris-HCl, (Blue Cap) pH … can diabetes cause low hemoglobinWebBioFact 2X Multi-Star PCR Series. BioFact H-Star Dfree Taq DNA Polymerase. BioFact Genomic DNA Prep Kit [Column type] BioFact RT Series. BioFact PCR Purification Kit. BioFact Real-time PCR Series. BioFact PCR Purification Solution. BioFact Gel & PCR Purification System. BioFact Plasmid Prep Solution. fish on air fryerWebFigure 5. Uncompromised specificity of Platinum Taq DNA polymerase, DNA-free, as compared to competitor. Performance of Platinum Taq DNA polymerase, DNA-free (blue curves), and Promega GoTaq MDx Hot start Polymerase (green curves) in qPCR assays were evaluated using primers targeting E. coli 16S rRNA gene and varying amount of E. … fish on a pole pngWebBIOFACT Co.,Ltd. - Korea supplier of Taq, PCR, DNA Prep Kits, DNA Amplification, DNA Purification, DNA isolation, DNA Clean Up, Taq DNA Polymerase, Multiplex PCR ... fishon archWebOverview. 250 U (5 U/µl), hot-start polymerase for PCR and real-time PCR, includes 1.25 ml each 10x PCR buffer and 50 mM MgCl 2. List Price: fish on a plateWebApr 15, 1989 · From the predicted 832-amino acid sequence of the Taq DNA polymerase gene, Taq DNA polymerase has significant similarity to E. coli DNA polymerase I. We subcloned and expressed appropriate portions of the insert from a lambda Ch35 library candidate to yield thermostable, active, truncated, or full-length forms of the protein in E. … fishon app