Cytochrome c aptamer
WebDec 30, 2014 · We have developed an aptameric nanosensor for fluorescence activation imaging of cytochrome c (Cyt c). Fluorescence imaging tools that enable visualization … WebFeb 18, 2024 · Cytochrome c (cyt c) is a small hemeprotein (molecular weight of 12 kDa, and size approximately 5 nm), that serves as electron carrier in a respiratory chain of the …
Cytochrome c aptamer
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WebDec 17, 2024 · Cytochrome c (Cyt c), a heme protein, can be a potential biomarker for cell-apoptosis or even cancer diagnosis. In this work, a simple, rapid, sensitive and selective label-free assay for... WebThe optical or electrochemical probes are functionalized with photoresponsive o-nitrobenzylphosphate ester caged Cyt. c aptamer constituents. These are uncaged by light stimuli at single cell subdomains, allowing the spatiotemporal detection of Cyt. c through the formation of Cyt. c/aptamer complexes at non-apoptotic or apoptotic constraints.
WebAug 15, 2024 · With modifications to an ultra-sensitive bio-barcode (BBC) assay, we have developed a next generation aptamer-based bio-barcode (ABC) assay to detect cytochrome-c (Cyto-c), a cell death marker released from cancer cells, for anti-cancer drug screening. An aptamer is a short single-stranded DNA selected from a synthetic … WebJun 18, 2024 · MF-3WJ consisted of three arms, namely, an IFN-γ aptamer sequence, a thiol group, and a C–C mismatch sequence. Ag + was inserted between the C–C mismatched sequences to stabilize the double strand, and Ag + was used as a redox label . The reliability of the electrochemical biosensor was secured by measuring seven …
Webaptamer-mediated phosphorescent sensor with high specificity and operational simplicity can effectively avoid the interference of scattering light from complex substrates. Our findings offer a new clue for building biosensors based on QDs and aptamers. 1. Introduction Cytochrome c (Cyt c) is a hemachrome-containing metal micromolecular ... WebOct 15, 2013 · Based on a recently reported ultra-sensitive bio-barcode (BBC) assay, we have developed an aptamer-based bio-barcode (ABC) alternative to detect a cell death …
WebFeb 1, 2014 · In this work, we present a label -free impedimetric aptasensor for the recognition of cytochrome c (Cyt c) at a pM concentration level based on an epoxy-graphite composite electrode.The technique employed for the detection of the protein Cyt c is electrochemical impedance spectroscopy (EIS). Detection occurs when the protein …
WebMay 14, 2010 · This cyto- c specific aptamer was then used in the modified BBC assay to detect the cyto- c in the culture medium released from apoptotic cells. In the ABC assay, because of the small size and its immuno-specificity, aptamer was used in the assay for both recognition and readout reporting. how to say please let me know your thoughtsWebApr 12, 2024 · This method exhibited significant selectivity against target among ten coexisting proteins (fibrinogen, hemoglobin, cytochrome C, serum albumin, alkaline … northlander train scheduleWebDec 10, 2024 · Selective and sensitive detection of human activated protein C (APC) was performed herein by using carbon nanofiber (CNF) and ionic liquid (IL) composite modified pencil graphite electrode (PGE) and electrochemical impedance spectroscopy (EIS) technique. A carbon nanomaterial-based electrochemical aptasensor was designed and … how to say please let me go in spanishWebHerein, mitochondria-targeting Pt nanoclusters were synthesized using cytochrome c aptamer (CytcApt) as template. The obtained CytcApt-PtNCs can produce ⋅ OH by H 2 O 2 under the acidic conditions. Moreover, CytcApt-PtNCs could kill 4T1 tumor cells in a pH-dependent manner, but had no side effect on normal 293T cells. northlander trailers for saleWebJul 13, 2024 · The cytochrome complex or cytochrome c (Cyt c) is a small water-soluble hemeprotein with a heme core in the middle of its 3D structure. The molecules of this … northlander tiny homesWebJun 24, 2015 · Cytochrome c (Cyt c) is a heme-containing metalloprotein located in the intermembrane space of mitochondria. It plays a central role in electron transport chain and it is also an intermediate in apoptosis. When mitochondria are injured under pathological conditions, Cyt c is released into the cytosol of the cell. northlander sword prototypeWebApr 12, 2024 · This method exhibited significant selectivity against target among ten coexisting proteins (fibrinogen, hemoglobin, cytochrome C, serum albumin, alkaline phosphatase, T4 polynucleotide kinase in blood serum, and amylase, sialomucin, lysozyme, and immunoglobulin G usually discovered in saliva), attributed to the highly selective … how to say please speak slowly in spanish