Immortalized human hepatocytes
Witryna4 cze 2024 · Human primary hepatocytes (PHs) are critical to studying liver functions, drug metabolism and toxicity. PHs isolated from livers that are unacceptable for transplantation have limited expansion and culture viability in vitro, in addition to rapidly deteriorating enzymatic functions. The unsuitability of immortalized hepato … WitrynaAn immortalized human hepatocyte cell line, HepLi5, was established via transfection of Simian virus 40 large T antigen (SV40 LT) into primary human hepatocytes. The …
Immortalized human hepatocytes
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Witryna13 kwi 2024 · Functional human hepatocytes as surrogates for PHH have been generated in vitro using stem cell-based techniques, ... HNF4A, and HNF1A … WitrynaMethods: Human hepatocytes were immortalized with a retroviral vector SSR#197 expressing catalytic subunit of human telomerase reverse transcriptase (hTERT) and enhanced green fluorescent protein (EGFP) cDNAs flanked by a pair of loxP recombination targets. Then, Tamoxifen-dependent Cre recombinase was expressed …
WitrynaTo develop a murine model for HBV production, we established an immortalized, cloned liver cell line by transferring the Simian Virus 40 Large T-Antigen into primary human … WitrynaImmortalized hepatocytes (Fa2N-4 cells) have been proposed as a … Cytochrome P4503A4 (CYP3A4) is the principal drug-metabolizing enzyme in human liver. Drug-drug interactions (DDIs) caused by induction of CYP3A4 can result in decreased exposure to coadministered drugs, with potential loss of efficacy.
WitrynaA breakthrough in cell biology research Human telomerase reverse transcriptase (hTERT)–immortalized primary cells represent a breakthrough in cell biology research that combines the in vivo nature of primary cells with the traditional cell line's ability to survive continuously in vitro. Witryna15 wrz 2015 · We sought to develop and characterize a novel human liver cell line (LIV0APOLY) to establish a model of lipid accumulation using a physiological mixture of fatty acids under low- and high-glucose conditions. LIV0APOLY cells were compared with a well-established cell line (HepG2) and, where possible, primary human hepatocytes.
WitrynaImmortalized Human Hepatocytes for In Vitro Research and Drug Development. Immortalized Fa2N-4 hepatocytes allow you to compare results using hepatocytes …
Witryna9 mar 2024 · Moreover, human-derived TERT (hTERT) gene has also been used for inducing immortalized hepatocytes . In addition to the function of hTERT to maintain telomere length, it is reported to bind to transcription factors such as p65 or β -catenin and to regulate gene expression related to tumorigenesis [ 31 ]. can sharks can eat peopleWitryna30 wrz 2011 · The development of continuous non-tumorous cell line steadily containing hepatocyte phenotypes would substitute the primary hepatocytes for these studies. … can sharks drown if they stop movingWitryna8 lut 2015 · This study aimed to establish immortalized human HSC using the simian virus 40 large T antigen (SV40LT) for applications in co-culturing with hepatocytes … flannel shirts no patternsWitrynaImmortalized differentiated liver cell lines capable of continuous proliferation, and expressing stable liver-specific functions, would be valuable for in vitro toxicity testing … flannel shirts on carsflannel shirts on a dateWitryna1 paź 2014 · Conditional immortalization enables the development of growth-controlled cell lines. At least three strategies have been reported to conditionally immortalize … can sharks eat eelsWitrynaA variety of immortalized human hepatocytes have already proven useful as tools for liver-based in vitro testing and fundamental research purposes. The present … can sharks eat chocolate