WebCaracterización de la secuencia PR77 con función dual promotor-ribozima en el elemento móvil L1TC de Trypanosomo cruzi: análisis de la interacción de las actividades transcriptasa inversa y chaperona de ácidos nucleicos de L1Tc WebA, B and C represent three different reading frames and also contain slightly different restriction sites. pAcHLT-B (8.1 kbp) polyhedrin ATG, GST, BamH I, 6x His tag, Protein kinase A site, Thrombin cleavage-Xho I, EcoR I, Stu I, Nco I, Sac I, Not I, Sse8387 I, Pst I, Kpn I, Sma I, Bgl II pAcHLT-C (8.1 kbp) polyhedrin
Common vectors for protein expression - CSH Protocols
WebThe Baculovirus Expression Vector System is based on the introduction of a foreign gene into a nonessential region of the viral genome via homologous recombination with a transfer vector containing the cloned gene; an event that occurs in the co-transfected insect cells. WebSep 14, 2010 · into baculovirus vector, pAcHLT-C (Pharmingen) via PCR amplifi-cation at XhoI and NcoI sites. Cell culture. Human embryonic kidney (HEK)293, HEK293T, and ... methanol at 4°C at 40 V overnight, and the membranes were incu-bated in TBST (containing 100 mM Tris-Cl, pH 7.5, 150 mM NaCl, henry rollins tv shows
Purification and Cloning of a Brefeldin A-inhibited Guanine …
WebThe reaction is incubated at 37 °C for 30 ... (Spodoptera frugiperda ovary) cells are cotransfected with bacu-lovirus containing the BIG1 cDNA construct in pAcHLT-C and Baculogold DNA (PharMingen). To purify the expressed protein, cells from a 75-mm 2 flask are sedimented by centrifugation (∼1000 rpm, ... WebpAcHLT C 8.1kb A m p R p U C O r i f 1 O r i O R F 1 6 2 9 Nde l Xho l EcoR l Nco l Sac l Not l Pst l Kpn l Sma l Bgl ll O R F 6 0 3. Polyhedrin promoter His tag - GTTTTGTAAT … WebApr 30, 1999 · BIG2-Full, excised with Nde I and Not I, was ligated to the baculovirus transfer vector pAcHLT-C with the His 6 sequence encoded at its NH 2 terminus. A sample (2 μg) and 0.5 μg of BaculoGold DNA (Pharmingen) were mixed with 2 × 10 6 Sf9 cells in 3 ml of TNM-FH Insect medium (Pharmingen). henry rollins tv show hosted what show