Phosphinothricin-resistant

WebSep 12, 2015 · In this study, we successfully performed Agrobacterium-mediated genetic transformation of Salvia miltiorrhiza and produced herbicide-resistant transformants. Leaf discs of S. miltiorrhiza were infected with Agrobacterium tumefaciens EHA105 harboring pCAMBIA 3301. The pCAMBIA 3301 includes an intron-containing gus reporter and a bar … WebApr 11, 2024 · Pioneer Hi-Bred International (Pioneer) has submitted a petition (APHIS Petition Number 20–203–01p) to APHIS seeking a determination of nonregulated status for DP23211 maize (corn), which was developed using genetic engineering for insect resistance to western corn rootworm and contains the gene that codes for the phosphinothricin ...

Non-specific activities of the major herbicide-resistance gene BAR

WebJan 1, 2010 · The resistance to phosphinothricin is stable and is inherited by the R1 progeny as a single dominant Mendelian trait. The transgenic plants are highly resistant to the broad spectrum herbicide ... WebOct 23, 2015 · L-Phosphinothricin [PPT; glufosinate; L-homoalanine-4-yl- (methyl)phosphinic acid] is the active ingredient of the nonselective herbicide BASTA (AgrEvo, Frankfurt am Main, Germany), which is a nonselective, postemergence herbicide used for weed control in orchards and vineyards 1. impact blog editor https://asadosdonabel.com

Resistance to glufosinate is proportional to

WebOct 4, 2009 · The transgenic plants were resistant to phosphinothricin up to 200 mg/l, … WebPhosphinothricin acetyltransferase (PAT) assays confirmed the expression of the bar gene in plants obtained from PPT-resistant calli. The only exceptions were two plants obtained from the same callus that had multiple copies of the bar gene integrated into their genomes. The transgenic status of the plants was varified by Southern blot analysis. WebJan 27, 2024 · The fast spread of bacteria that are resistant to many classes of antibiotics (multidrug resistant) is a global threat to human and animal health with a worrisome scenario ahead. Novel therapeutical strategies are … impact blood center

Non-specific activities of the major herbicide-resistance gene BAR

Category:Efficient expression of heterologous genes by the introduction of …

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Phosphinothricin-resistant

Efficient expression of heterologous genes by the introduction of …

WebResistance plasmids include pGreenII 0229 and pGreenII 0229 62-SK. pGreenII 0229 is derived from pGreenII 0000, a nos-bar cassette has been inserted into the HpaI site of the left border, providing resistance to bialaphos or phosphinothricin during plant transformation selection. pGreenII 0229 62-SK is derived from pGreenII 0229, the LacZ … WebApr 11, 2024 · The Animal and Plant Health Inspection Service (APHIS) issued a final rule, published in the Federal Register on May 18, 2024 (85 FR 29790-29838, Docket No. APHIS-2024-0034) \1\, revising 7 CFR part 340; however, the final rule is being implemented in phases. The new Regulatory Status Review (RSR) process, which replaces the petition for ...

Phosphinothricin-resistant

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WebPhosphinothricin is not an effective antibiotic because of its inability to be readily … WebWe have previously transformed rice (Oyrza sativa L.) with the maize ribosome-inactivating protein b-32 gene (Zmcrip3a) and the phosphinothricin resistance gene (bar). In the present study, Southern blot hybridization analysis of 56 primary fertile transformants resulted in distinct band patterns, indicating that all the transformants had been generated by …

WebNov 6, 2006 · Selection for kanamycin-, phosphinothricin- and hygromycin B-resistance commonly takes 7-10 d and high seedling density and fungal contamination may result in failure to recover transformants. Results: A method for identifying transformed seedlings in as little as 3.25 d has been developed. WebL-phosphinothricin (PPT) is a potent inhibitor of the biosynthetic enzyme glutamine …

WebJan 1, 2008 · The use of phosphinothricin resistance as selectable marker for genetic . transformation of grapevine. R. J ARDAK-J AMOUSSI 1), B. B OUAMAMA 1), A. M LIKI 1), A. G HORBEL 1) and G. M. R EUSTLE 2) WebLibertyLink (®) cotton cultivars are engineered for glufosinate resistance by …

WebThese results indicated that the extra intron at the 5 0 end of the phosphinothricin-resistance gene is required for its efficient expression in G. lucidum. To confirm these results, we also ...

WebNov 27, 2024 · In the 1980s, the bialaphos resistance ( BAR) gene and its closely related … impact blood loginWebresistance of expiants to, phosphinothricin is presented. Key words: Ammonium - Carbon source - Glufosinate - Glutamine - Metabolic activity - Phosphinothricin Introduction Selectable marker genes are still a necessity in plant trans formation technology. In the presence of a selective agent, only those cells that are transformed and express the list qualities of a good friendWebNov 1, 1996 · v ol. 62, 1996 resistance to phosphinothricin in c. reinhardtii 3835 at concentrations as low as 0.27 mM (50 m g/ml), at which both GS activity and ammonium utilization were completely abol- impact bloodstain patternWebA bioassay was developed for identifying transgenic wheat plants based on De Block et al.'s [8] ammonium-multiwell assay which allows qualitative and quantitative evaluation of the expression of the enzyme phosphinothricin acetyl transferase (PAT). Important parameters in the assay are the use of young leaf tissues, short incubation period (6 h) and a high … impact blood patternWebJul 14, 2024 · In the current study, a chloroplast transformation vector harboring the phosphinothricin resistance gene (bar) as a selectable marker was established, and two short peptide-encoding genes (ant1 and ant2) driven by the endogenous psbA and rbcL promoters were cloned in this vector. The recombinant plasmid was transformed using a … impact blueprint frameworkWebJan 1, 2008 · Phosphinothricin resistance as selectable marker for genetic transformation … impact blue harleyWebA gene which confers resistance to the herbicide bialaphos (bar) has been characterized. The bar gene was originally cloned from Streptomyces hygroscopicus, an organism which produces the tripeptide bialaphos as a secondary metabolite. Bialaphos contains phosphinothricin, an analogue of glutamate which is an inhibitor of glutamine synthetase. impact blood donation